2ml EDTA Tube Purple Top Vacuum Blood Collection EDTA Tube K3 Coagulation Tests
2ml EDTA Tube Purple Top Vacuum Blood Collection EDTA Tube K3
1. Product Introducation:
EDTA prevents clotting by chelating calcium, an essential component of coagulation. This is your basic hematology tube (by which I mean identifying and counting blood cells, blood typing, etc).
Plasma stored from EDTA-treated blood can also be used to measure most proteins, and genetic material can easily be stored from EDTA buffy coats (the interface between the red cells and the plasma after centrifugation, containing white cells and platelets). Note: these tubes contain either K2EDTA or K3EDTA.
2. Vacutainer EDTA tubes FAQ
Q: What does EDTA stand for and how does the anticoagulant work?
A: EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium in the blood and keeping the blood from clotting
Q: What is the concentration of EDTA in the lavender and pink stoppered tubes?
A: The nominal EDTA concentration is 1.8mg EDTA per milliliter of blood.
Q: Why do some Vacutainer lavender top blood collection tubes contain K2EDTA and others contain K3EDTA?
A: Vacutainer Plus Blood Collection Tubes contain K2EDTA, which is spray-dried to the walls of the tube. K2EDTA is recommended by the CLSI (CLSI guideline - Evacuated Tubes and Additives for Blood Specimen Collection-Fifth Edition H1-A5, 2003) and the ICSH (International Council for Standardization in Hematology). Vacutainer glass blood collection tubes contain K3EDTA, the liquid form of EDTA.
Vacutainer/sample tube types for venipuncture/phlebotomy edit | Tube cap color or type | Additive | Usage and comments |
| Blood culture bottle | Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms | Usually drawn first for minimal risk of contamination.[8] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms.[9] |
| Light blue | Sodium citrate (anticoagulant) | Coagulation tests such as prothrombin time (PT) and partial thromboplastin time (PTT) and thrombin time (TT). Tube must be filled 100%. |
| Plain red | No additive | Serum: Total complement activity, cryoglobulins |
| Gold (sometimes red and grey "tiger top"[10]) | Clot activator and serum separating gel[11] | Serum-separating tube: Tube inversions promote clotting. Most chemistry, endocrine and serology tests, including hepatitis and HIV. |
| Dark green | Sodium heparin (anticoagulant) | Chromosome testing, HLA typing, ammonia, lactate |
| Mint green | Lithium heparin (anticoagulant) | Plasma. Tube inversions prevent clotting |
| Lavender ("purple") | EDTA (chelator / anticoagulant) | Whole blood: CBC, ESR, Coombs test, platelet antibodies, flow cytometry, blood levels of tacrolimus and cyclosporin |
| Pink | EDTA (chelator / anticoagulant) | Blood typing and cross-matching, direct Coombs test, HIV viral load |
| Royal blue | EDTA (chelator / anticoagulant) | Trace elements, heavy metals, most drug levels, toxicology |
| Tan | EDTA (chelator / anticoagulant) | Lead |
| Gray | | Glucose, lactate[13] |
| Yellow | Acid-citrate-dextrose A (anticoagulant) | Tissue typing, DNA studies, HIV cultures |
| Pearl ("white") | Separating gel and (K2)EDTA | PCR for adenovirus, toxoplasma and HHV-6 |
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2ml Purple Top Vacuum Blood Collection EDTA Tube K3 Coagulation Tests Images
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